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HG SYBR Green miRNA荧光定量PCR试剂盒应用Peter改良的miR-Q技术，采用特异性的上下游引物对反转录所得miRNA的cDNA产物进行定量检测【1】。不同于其它公司的单条特异性引物扩增方法，HaiGene采用双向特异性扩增引物（原理如图1），从而极大提高了miRNA的检测特异性，其可以区分极高相似度的miRNA分子、减少非特异性扩增（图2，3所示）。该miRNA荧光定量PCR试剂盒对可以对任何miRNA分子进行检测。HG miRNA荧光定量PCR试剂盒共有一万余种，试剂盒编号为APXXXXX，每一个miRNA分子对应一个检测试剂盒，可于HG SYBR Green miRNA qPCR kit.xls中查询。如果您研究的miRNA分子不在我们的列表中，请来信咨询，我们将及时给您优化设计。该试剂盒中的每一对引物均经过设计优化，确保扩增效率和特异性。使用该方法进行miRNA定量检测已被大量文献所采用【2～11】。 |
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| 避光置于-20°C，可保存2年；避免反复冻融。 | ||||||||||||||||
常用miRNA内参荧光定量PCR试剂盒 |
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| 注：其它miRNA荧光定量检测试剂盒请查询HG SYBR Green miRNA qPCR kit.xls。 | ||||||||||||||||
参考文献(其中3-11为使用该方法发表的文献） |
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| [1] Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers. BMC Biotechnol. 2011 Jun 25; 11: 70. PMC3135530. [2] Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling. J Anim Sci Biotechnol. 2012 PMID:22958414. [3] Reliable reference miRNAs for quantitative gene expression analysis of stress responses in Caenorhabditis elegans. BMC Genomics. 2014. PMID:24656064. [4] Profiling microRNAs in lung tissue from pigs infected with Actinobacillus pleuropneumoniae. BMC Genomics. 2012. PMID:22953717. [5] The role of viral and host microRNAs in the Aujeszky's disease virus during the infection process. PLoS One. 2014. PMID:24475202. [6] MicroRNA polymorphisms and environmental smoke exposure as risk factors for oesophageal squamous cell carcinoma. PLoS One. 2013.PMID:24205249. [7] Plasma proANP and SDMA and microRNAs are associated with chronic mitral regurgitation in a pig model. Endocr Connect. 2013.PMID:24029364. [8] Advances in microRNA experimental approaches to study physiological regulation of gene products implicated in CNS disorders. Exp Neurol. 2012. PMID:22245616. [9] Maternally deposited germline piRNAs silence the tirant retrotransposon in somatic cells. EMBO Rep. 2013. PMID:23559065. [10] miRNA expression profile analysis in kidney of different porcine breeds. PLoS One. 2013. PMID:23372853. [11] Determination of reference microRNAs for relative quantification in porcine tissues. PLoS One. 2012. PMID:22970213. |
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